Strategies to design photoswitchable fluorescent proteins and their application in nanoscopy

Lecturer:

Course typ:

Blockveranstaltung

Description:

The course will be given by Stefan Jakobs (sjakobs@gwdg.de) and Nickels Jensen (njensen@gwdg.de).

GFP-like fluorescent proteins are powerful tools to study protein dynamics in living cells. The actual properties of the fluorescent proteins may be dramatically altered by slight changes in their amino acid sequences. This short practical course will cover several basic methods for targeted and random mutagenesis based on PCR. We will use the coding sequences of switchable fluorescent proteins as templates. The mutagenized proteins will be analyzed for variants exhibiting different properties, using fluorescence activated cell sorting (FACS) and an automated screening microscope setup. The RSFPs will be used for live-cell super-resolution microscopy.

Place:

unspecified

Semester:

WiSe 2017/18

Times:

Dates on Tuesday, 17.10.2017 - Wednesday, 18.10.2017 09:15 - 23:59
First appointment: Tuesday, 17.10.2017 09:15 - 23:59

Course number:

340226

Participants:

Target Group: GGNB doctoral students

Learning organisation:

The course will take place at the MPI for Biophysical Chemistry, Department of NanoBiophotonics, Tower 2, 2nd floor.

Area classification:

Vorlesungsverzeichnis WiSe 2017/2018 > Forschungseinrichtungen > Göttinger Graduiertenschulen > Georg-August University School of Science (GAUSS) > Göttinger Graduiertenschule für Neurowissenschaften und molekulare Biowissenschaften (GGNB) > Methods Courses > Molecular Biology & Genetics

ECTS credit points:

1.0

Further information from Stud.IP about this course

Home institute: Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Participants registered in Stud.IP: 2
Number of postings in Stud.IP forum: 2