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Analysis of miRNA posttranscriptional regulation by Flow Cytometry
Lecturer:
N. N.
Course typ:
Blockveranstaltung
Description:
The course will be given by Nicolas Lemus-Diaz: nlemus@dpz.eu


MicroRNAs (miRNA) are critical regulators of cellular fate and function, controlling mRNA levels through sequence dependent post-transcriptional repression. Briefly miRNA promote degradation on RNA targets upon sequence complementarity.

 To study endogenous miRNA function we designed an assay based on two fluorescent proteins, where one is a reference, and the other contains a region complementary to a miRNA sequence. Allowing single cell analysis by flow cytometry, furthermore using a  molecular titration model for miRNA-driven gene repression and the R environment increasing the resolution and understanding of miRNAs induced regulation.

During the course, the participants will have hands-on on transfection, FACS measurement and data analysis.

The course includes basic transfection techniques, flow cytometry, and data analysis in R.

Output results are described in the following research paper:

Lemus-Diaz, N. et al. Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system. Scientific reports 7, 45197, doi:10.1038/srep45197 (2017).
Place:
unspecified
Semester:
SoSe 2018
Times:
Dates on Wednesday, 18.07.2018 - Friday, 20.07.2018 09:00 - 23:59
First appointment: Wednesday, 18.07.2018 09:00 - 23:59
Course number:
340331
Participants:
Target group: GGNB and GAUSS doctoral students.
Learning organisation:
The course will take place at the German Primate Center.
Area classification:
Vorlesungsverzeichnis SoSe 2018 > Forschungseinrichtungen > Göttinger Graduiertenschulen > Georg-August University School of Science (GAUSS) > Göttinger Graduiertenschule für Neurowissenschaften und molekulare Biowissenschaften (GGNB) > Methods Courses > Molecular & Cellular Neuroscience, Electrophysiology
ECTS credit points:
1.5
Further information from Stud.IP about this course
Home institute: Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Participants registered in Stud.IP: 1
Number of postings in Stud.IP forum: 2