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Analysis of miRNA posttranscriptional regulation by Flow Cytometry |
Lecturer: |
N. N. |
Course typ: |
Blockveranstaltung |
Description: |
The course will be given by Nicolas Lemus-Diaz: nlemus@dpz.eu MicroRNAs (miRNA) are critical regulators of cellular fate and function, controlling mRNA levels through sequence dependent post-transcriptional repression. Briefly miRNA promote degradation on RNA targets upon sequence complementarity. To study endogenous miRNA function we designed an assay based on two fluorescent proteins, where one is a reference, and the other contains a region complementary to a miRNA sequence. Allowing single cell analysis by flow cytometry, furthermore using a molecular titration model for miRNA-driven gene repression and the R environment increasing the resolution and understanding of miRNAs induced regulation. During the course, the participants will have hands-on on transfection, FACS measurement and data analysis. The course includes basic transfection techniques, flow cytometry, and data analysis in R. Output results are described in the following research paper: Lemus-Diaz, N. et al. Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system. Scientific reports 7, 45197, doi:10.1038/srep45197 (2017). |
Place: |
unspecified |
Semester: |
SoSe 2018 |
Times: |
Dates on Wednesday, 18.07.2018 - Friday, 20.07.2018 09:00 - 23:59 First appointment: Wednesday, 18.07.2018 09:00 - 23:59 |
Course number: |
340331 |
Participants: |
Target group: GGNB and GAUSS doctoral students. |
Learning organisation: |
The course will take place at the German Primate Center. |
Area classification: |
Vorlesungsverzeichnis SoSe 2018 > Forschungseinrichtungen > Göttinger Graduiertenschulen > Georg-August University School of Science (GAUSS) > Göttinger Graduiertenschule für Neurowissenschaften und molekulare Biowissenschaften (GGNB) > Methods Courses > Molecular & Cellular Neuroscience, Electrophysiology |
ECTS credit points: |
1.5 |
Further information from Stud.IP about this course |
Home institute: Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) Participants registered in Stud.IP: 1 Number of postings in Stud.IP forum: 2 |