Using biosensors to study cellular redox processes

Lecturer:

Course typ:

Blockveranstaltung

Description:

First a theoretical introduction is given about genetically encoded redox biosensors and their application in the life sciences. Second, in the practical part we will mainly focus on the protocol for isolation of cardiomyocytes from adult mouse heart by Langendorff´s perfusion system followed by the redox measurements required to obtain the glutathione redox potential (EGSH) using the Grx1-roGFP2 biosensor. The possible applications of this sensor model along with the associated limitations will also be discussed along with the experimental demonstration of the technique.

Place:

unspecified

Semester:

SoSe 2018

Times:

Dates on Monday, 24.09.2018 08:30 - 23:59
First appointment: Monday, 24.09.2018 08:30 - 23:59

Course number:

340340

Participants:

Target group: GGNB and GAUSS doctoral students.

Learning organisation:

The course will be given by Dörthe Katschinski and Maithily Nanadikar Dörthe Katschinski: doerthe.katschinski@med.uni-goettingen.de Maithily Nanadikar: maithily.nanadikar@med.uni-goettingen.de The course will take place at the Institute of Cardiovascular Physiology, University Medical Center, Humboldtallee 23, 37073 Göttingen.

Area classification:

Vorlesungsverzeichnis SoSe 2018 > Forschungseinrichtungen > Göttinger Graduiertenschulen > Georg-August University School of Science (GAUSS) > Göttinger Graduiertenschule für Neurowissenschaften und molekulare Biowissenschaften (GGNB) > Methods Courses > Biochemistry

ECTS credit points:

0.5

Further information from Stud.IP about this course

Home institute: Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Participants registered in Stud.IP: 1
Number of documents in the Stud.IP download area: 1