Vorlesung mit Übung: Molecular Techniques in Phytopathology - Details

Vorlesung mit Übung: Molecular Techniques in Phytopathology - Details

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General information

Course name Vorlesung mit Übung: Molecular Techniques in Phytopathology
Subtitle
Course number 740027
Semester SoSe 2023
Current number of participants 4
maximum number of participants 12
Home institute Pflanzenpathologie und Pflanzenschutz
Courses type Vorlesung mit Übung in category Teaching
First date Monday, 04.09.2023 08:00 - 17:00, Room: (Großprakti (Pflanzenpathologie-Institutsgebäude))
Type/Form
Participants AGRNP MSC ab 1
Pre-requisites Anmeldung erforderlich, beschränkte Teilnehmerzahl

Rooms and times

(Großprakti (Pflanzenpathologie-Institutsgebäude))
Monday, 04.09.2023 - Friday, 08.09.2023, Monday, 11.09.2023 - Friday, 15.09.2023 08:00 - 17:00
(L07 (Pflanzenpathologie-Institutsgebäude))
Monday, 11.09.2023 - Friday, 15.09.2023 08:00 - 17:00

Fields of study

Module assignments

Comment/Description

Contents:
With the help of laboratory experiments, basic molecular techniques will be taught,
including the isolation of plasmids and total DNA as well es DNA fragments from
electrophoretic agarose gels, cloning of PCR products (enzymatic modification, ligation),
transformation and in vivo amplification of plasmids, DNA blotting, labeling of DNA
probes for hybridization with nonradioactive labels (DIG-dUTP), Southern hybridization
and detection of hybridized probes by chemiluminescence, ITS-RFLP-analysis of
pathogens of oilseed rape, real-time PCR for mycotoxin-producing pathogens of cereals.
In accompanying lectures, aspects of nucleic acid and protein chemistry relevant
for analytical techniques will be explained. Technical solutions for specific scientific
questions will be presented and discussed.

Learning outcome, core skills:
Technical skills in nucleic acid nucleic acid analysis techniques; ability to select adequat
methods for a research project. Presentation and interpretation of the results.

Admission settings

The course is part of admission "Beschränkte Teilnehmendenanzahl: Molecular Techniques in Phytopathology".
The following rules apply for the admission:
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 18.04.2023 on 23:59. Additional seats may be available via a waiting list.