Veranstaltungsdaten

Beschreibung: Note: We offer an introductory command line course (course number: A 306), which is beneficial for this course.

In this 3 day course we will give a general introduction to recent advances in sequencing technologies (next generation sequencing, NGS) and the respective fields of application. In hands-on training sessions the participants will be guided through typical analysis pipelines for differential gene expression, de novo assembly of transcriptomes and ChIP-seq datasets.

1st day - Topic: de novo Assembly

Room: Seminar room -1.105, GZMB, Justus-von-Liebig-Weg 11

9:15-10:00 Lecture: Introduction to NGS technologies and applications (Nico Posnien)

10:05-10:50 Lecture: Introduction to de-novo assembly (DNA, RNA) (Nico Posnien)

10:55:11:40 Lecture: Basics of graph theory (DeBruijn Graphs, etc) (Tim Beißbarth)

11:40-12:00 Discussions/Questions

12:00-13:30 Lunch

Room: CIP room -1.201B, GZMB, Justus-von-Liebig-Weg 11

13:30-16:30 Practical part: de-novo Transcriptome Assembly (Nico Posnien)

2nd day - Topic: RNA-Seq

Room:Seminar room -1.105, GZMB, Justus-von-Liebig-Weg 11

9:15-10:00 Lecture: Mapping Algorithms (Maksims Fiosins)

10:05-10:50 Lecture: Statistical analysis of RNA-seq data (Tim Beißbarth)

10:55-11:40 Lecture: Quality control, databases and tools for annotation of NGS data (Maksims Fiosins)

11:40-12:00 Discussion/Questions

12:00-13:30 Lunch

Room:CIP room -1.201B, GZMB, Justus-von-Liebig-Weg 11

13:30-16:30 Practical part: RNA-seq Mapping and Counting (Tim Beißbarth)

3rd day - Topic: ChIP-Seq

Room:Seminar room -1.105, GZMB, Justus-von-Liebig-Weg 11

9:15-10:00 Lecture: Introduction to ChIP-seq (Nico Posnien)

10:05-10:50 Lecture: Peak-Detection and algorithms in ChIP-seq analysis (Maksims Fiosins)

10:55-11:40 Lecture: Motif search (Tim Beißbarth)

11:40-12:00 Discussion/Questions

12:00-13:30 Lunch

Room:CIP room -1.201B, GZMB, Justus-von-Liebig-Weg 11

13:30-16:30 Practical part: ChIP-Seq Analysis (Maksims Fiosins)

Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.