Blockveranstaltung: Investigating macromolecular complexes by density gradient centrifugation - Details

Blockveranstaltung: Investigating macromolecular complexes by density gradient centrifugation - Details

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General information

Course name Blockveranstaltung: Investigating macromolecular complexes by density gradient centrifugation
Subtitle
Course number 340415
Semester SoSe 2020
Current number of participants 1
maximum number of participants 2
Home institute Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
participating institutes Georg-August University School of Science (GAUSS) (für Mathematik und Naturwissenschaften)
Courses type Blockveranstaltung in category Teaching
First date Thursday, 02.04.2020 10:00 - 23:59
Type/Form
Participants Target group: GGNB and GAUSS doctoral students.
Learning organisation The course will take place at Department of Cellular Biochemistry, Humboldtallee, 23 .

The course will be given by

Dr. Ricarda Richter-Dennerlein : ricarda.richter@med.uni-goettingen.de

Elisa Hanitsch: <elisa.hanitsch@med.uni-goettingen.de>

Franziska Nadler: franziska.nadler@med.uni-goettingen.de
ECTS points 1.0

Rooms and times

No room preference
Thursday, 02.04.2020 10:00 - 23:59
Friday, 03.04.2020 10:00 - 23:59

Comment/Description

Topic:

Density gradient centrifugation is widely used for separating cellular organelles or macromolecular complexes. During the course the biophysical background of ultracentrifugation will be discussed as well as experimental design (e.g. centrifugation speed, rotors, chemical compounds of gradient solutions) for specific scientific goals. As an example we will perform sucrose density gradient centrifugation with mitochondrial extracts to separate and analyze the mitochondrial ribosomal subunits.

 

Schedule:

Day 1:

- Theoretical introduction into sucrose gradient ultracentrifugation and Biocomp gradient station; Discussing the experimental workflow;

- Isolation of mitochondria from cultured human cells;

- Gradient preparation;

- Loading of mitochondrial lysates on a gradient and starting the ultracentrifuge.

 

Day 2:

- Fractionating the sucrose gradient using Biocomp gradient station;

- Discussing the results.

 

At the end of the course the participants should be able to plan and perform sucrose gradient ultracentrifugation and adjust experimental parameters for their specific scientific goals.

Admission settings

The course is part of admission "GGNB_Methods_2020_SoSe_February_April".
You can register and sign out for courses in April from 1-20 February. The slots will be assigned around 25 February.
In exceptional circumstances, you can still sign out before 29 February.
Your participation becomes binding on 1 March. Thereafter, cancellation is only possible with a) sick certificate, or b) statement by supervisor. If you do not provide this, you will be banned from courses for the current and next semester, as well as travel grants and other financial support.
By signing up you agree to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account.
The following rules apply for the admission:
  • At least one of these conditions must be fulfilled for enrolment:
    • Degree is Promotionsprogramm
    • Degree is Promotionsstudiengang
    • Degree is PromProgramm Cotutelle
    • Degree is Promotion m.Abschlussprfg
  • The enrolment is possible from 01.02.2020, 00:00 to 20.02.2020, 23:59.
  • At least one of these conditions must be fulfilled for enrolment:
    • Subject is PhysicsBiologComplexSyste
    • Subject is Geography
    • Subject is Mathematical Sciences
    • Subject is Geographie
    • Subject is Environmental Informatics
    • Subject is Biomol:Struc-Func-Dynamic
    • Subject is Chemie
    • Subject is Emerging Infect. Diseases
    • Subject is Microbiology-Biochemistry
    • Subject is Geoscience
    • Subject is Molekulare Biologie
    • Subject is Environmental Informatics
    • Subject is Behavior and Cognition
    • Subject is Biologische Diversität und Ökologie
    • Subject is Genome Science
    • Subject is Theoret Computat Neurosci
    • Subject is Genes in Development, Disease and Evolution
    • Subject is Catalysis Sust.Synt.CaSuS
    • Subject is Neurowissenschaften
    • Subject is IRTG 2172 - PRoTECT
    • Subject is Physik
    • Subject is Umweltinformatik
    • Subject is Molekulare Medizin
    • Subject is Systems Neuroscience
    • Subject is MolecularBiology of Cells
    • Subject is MetalSites i.Biomolecules
    • Subject is Computer Science -PCS-
    • Subject is SensoryMotor Neuroscience
    • Subject is BioNutz-MolwissBiotechnol
    • Subject is Materialforschung Holz
    • Subject is Biologie
    • Subject is MolecularPhysi oftheBrain
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 29.02.2020 on 23:59. Additional seats may be available via a wait list.
  • Enrolment is allowed for up to 2 courses of the admission set.
Assignment of courses:

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