General information

Course name	Blockveranstaltung: Super-resolution microscopy with DNA-PAINT and dSTORM. Super-resolution fluorescence lifetime imaging microscopy (SR-FLIM)
Subtitle
Course number	340406
Semester	WiSe 2022/23
Current number of participants	8
maximum number of participants	10
notwendige Teilnehmeranzahl	2
Home institute	Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Courses type	Blockveranstaltung in category Teaching
First date	Thursday, 03.11.2022 09:00 - 11:00
Type/Form
Participants	Target group: GGNB and GAUSS doctoral students.
Pre-requisites	The course is given by Nazar Oleksiievets: < nazar.oleksiievets@phys.uni-goettingen.de >

Lecturers

Rooms and times

No room preference: Thursday, 03.11.2022 09:00 - 11:00
Friday, 04.11.2022 09:00 - 11:00

Fields of study

Comment/Description

In this course, basics of fluorescence microscopy and super-resolution microscopy techniques will be covered, with the focus on single-molecule localization microscopy techniques. Two of the most important techniques, dSTORM and DNA-PAINT, will be discussed in detail. While dSTORM is more established and easy to implement, DNA-PAINT is a rapidly developing and versatile technique, capable of reaching superior resolution of single nanometers. Recent developments in cellular DNA-PAINT enable to exploit the full potential of DNA-PAINT for multiplexed imaging in fixed cells, then contributing to a deeper understanding of cellular organization and interplay. Advantages, limitations and experimental parameters optimization of dSTORM and DNA-PAINT will be discussed. In the practical part of the course, we will have a hands on the multiplexed DNA-PAINT imaging of fixed HeLa cells.

Course duration: two days. The course will include lecture/seminar/detailed overview of the experimental setups/hands-on session/data analysis training. Following topics will be included:

-          Introduction to optical microscopy and super-resolution techniques

-          dSTORM imaging

-          DNA-PAINT imaging (including multiplexing and variants of DNA-PAINT)

-          Microfluidics technology employed for multiplexing using Exchange DNA-PAINT

-          Multiplexed super-resolution imaging of fixed cells

-          Fluorescence lifetime imaging combined with super-resolution microscopy

-          data analysis of the collected data using different software packages

-          quantitative resolution estimation of the obtained super-resolution images

This two-day course will take place online via Zoom or at [F02.119, Third Institute of Physics, Faculty of Physics, Friedrich-Hund-Platz 1, 37077 Göttingen][1].

Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position.

[1]: https://lageplan.uni-goettingen.de/?ident=1440_1_2.OG_F.02.119

Admission settings

The course is part of admission "GGNB_Methods_2022_WiSe_September_November".

You can register and sign out for methods courses in November 2022 from 1-20 September 2022. The slots will be assigned around 25 September 2022.
In exceptional circumstances, you can still sign out until 30 September 2022.
Your participation becomes binding on 1 October 2022. Thereafter, cancellation is only possible via the GGNB Office with a) sick certificate, or b) statement by supervisor (for scientific reasons). If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates – the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation.
The following rules apply for the admission:

A defined number of seats will be assigned to these courses.
The seats in the affected courses have been assigned at 30.09.2022 on 23:59. Additional seats may be available via a wait list.
At least one of these conditions must be fulfilled for enrolment:
- Subject is Biologische Diversität und Ökologie
- Subject is Neurowissenschaften
- Subject is MolecularPhysi oftheBrain
- Subject is Microbiology-Biochemistry
- Subject is Materialforschung Holz
- Subject is IRTG 2172 - PRoTECT
- Subject is SensoryMotor Neuroscience
- Subject is BioNutz-MolwissBiotechnol
- Subject is Computer Science -PCS-
- Subject is Umweltinformatik
- Subject is Geography
- Subject is Psychologie
- Subject is Chemie
- Subject is Emerging Infect. Diseases
- Subject is Catalysis Sust.Synt.CaSuS
- Subject is MolecularBiology of Cells
- Subject is Molekulare Biologie
- Subject is MetalSites i.Biomolecules
- Subject is Environmental Informatics
- Subject is Mathematical Sciences
- Subject is Molekulare Medizin
- Subject is Geographie
- Subject is Geoscience
- Subject is Behavior and Cognition
- Subject is Theoret Computat Neurosci
- Subject is Environmental Informatics
- Subject is Physik
- Subject is Biomol:Struc-Func-Dynamic
- Subject is PhysicsBiologComplexSyste
- Subject is Cardiovascular Science
- Subject is Genome Science
- Subject is Genes in Development, Disease and Evolution
- Subject is Systems Neuroscience
- Subject is Biologie
Enrolment is allowed for up to 2 courses of the admission set.
At least one of these conditions must be fulfilled for enrolment:
- Degree is Promotion Cotutelle
- Degree is Promotionsstudiengang
- Degree is Promotionsprogramm
- Degree is PromStudiengang Cotutelle
- Degree is Promotion m.Abschlussprfg
- Degree is PromProgramm Cotutelle
The enrolment is possible from 01.09.2022, 00:00 to 20.09.2022, 23:59.

Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.

Blockveranstaltung: Super-resolution microscopy with DNA-PAINT and dSTORM. Super-resolution fluorescence lifetime imaging microscopy (SR-FLIM) - Details