Blockveranstaltung: Next Generation Sequencing (NGS) - Analysis of RNAseq and ChIPseq data - Details

Blockveranstaltung: Next Generation Sequencing (NGS) - Analysis of RNAseq and ChIPseq data - Details

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General information

Course name Blockveranstaltung: Next Generation Sequencing (NGS) - Analysis of RNAseq and ChIPseq data
Subtitle
Course number 340208
Semester WiSe 2022/23
Current number of participants 10
maximum number of participants 7
notwendige Teilnehmeranzahl 4
Home institute Georg-August University School of Science (GAUSS) (für Mathematik und Naturwissenschaften)
participating institutes Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Courses type Blockveranstaltung in category Teaching
First date Monday, 21.11.2022 09:15 - 11:00
Type/Form
Participants Target group: GGNB and GAUSS doctoral students.
Pre-requisites The course will take place in presence. Depending on the Covid situation, we may offer more places for the course (up to 15).

Rooms and times

No room preference
Monday, 21.11.2022 09:15 - 11:00
Tuesday, 22.11.2022 09:15 - 11:00
Wednesday, 23.11.2022 09:15 - 11:00

Fields of study

Comment/Description

In this 3 day course we will give a general introduction to recent advances in sequencing technologies (next generation sequencing, NGS) and the respective fields of application. In hands-on training sessions the participants will be guided through typical analysis pipelines for differential gene expression, de novo assembly of transcriptomes and ChIP-seq datasets.

 

Monday 21 November 2022 - Topic: de novo Assembly

Room: CIP, Medical Statistics, Humboldtallee 32

9:15-10:00            Lecture: Introduction to NGS technologies and applications (Nico Posnien)

10:05-10:50         Lecture: Introduction to de-novo assembly (DNA, RNA) (Nico Posnien)

10:55:11:40          Lecture: Basics of graph theory (DeBruijn Graphs, etc) (Tim Beißbarth)

11:40-12:00         Discussions/Questions

12:00-13:30         Lunch

Room: CIP, Medical Statistics, Humboldtallee 32

13:30-16:30         Practical part: de-novo Transcriptome Assembly (Nico Posnien)

 

Tuesday 22 November 2022 - Topic: RNA-Seq

Room: CIP, Medical Statistics, Humboldtallee 32

9:15-10:00            Lecture: Mapping Algorithms (Maksims Fiosins)

10:05-10:50         Lecture: Statistical analysis of RNA-seq data (Tim Beißbarth)

10:55-11:40         Lecture: Quality control, databases and tools for annotation of NGS data (Maksims Fiosins)

11:40-12:00         Discussion/Questions

12:00-13:30         Lunch

Room: CIP, Medical Statistics, Humboldtallee 32

13:30-16:30         Practical part: RNA-seq Mapping and Counting (Tim Beißbarth)

 

Wednesday 23 November 2022 - Topic: ChIP-Seq

Room: CIP, Medical Statistics, Humboldtallee 32

9:15-10:00            Lecture: Introduction to ChIP-seq (Nico Posnien)

10:05-10:50         Lecture: Peak-Detection and algorithms in ChIP-seq analysis (Maksims Fiosins)

10:55-11:40         Lecture: Motif search (Tim Beißbarth)

11:40-12:00         Discussion/Questions

12:00-13:30         Lunch

Room: CIP, Medical Statistics, Humboldtallee 32

13:30-16:30         Practical part: ChIP-Seq Analysis (Maksims Fiosins)

This 3-day course will take place from 21, 22 and 23 November 2022 at

[Computer Pool of the Dpt. of Medical Statistics, Humboldtallee 32][1]

[(Raum: 00-150/00-151][1])

Prof. Dr. Tim Beißbarth: Tim.Beissbarth@med.uni-goettingen.de

Dr. Nico Posnien: nposnie@gwdg.de



Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position.

[1]: https://lageplan.uni-goettingen.de/?ident=4395_1_EG_0.150

Admission settings

The course is part of admission "GGNB_Methods_2022_WiSe_September_November".
You can register and sign out for methods courses in November 2022 from 1-20 September 2022. The slots will be assigned around 25 September 2022.
In exceptional circumstances, you can still sign out until 30 September 2022.
Your participation becomes binding on 1 October 2022. Thereafter, cancellation is only possible via the GGNB Office with a) sick certificate, or b) statement by supervisor (for scientific reasons). If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates – the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation.
The following rules apply for the admission:
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 30.09.2022 on 23:59. Additional seats may be available via a wait list.
  • At least one of these conditions must be fulfilled for enrolment:
    • Subject is Biologische Diversität und Ökologie
    • Subject is Neurowissenschaften
    • Subject is MolecularPhysi oftheBrain
    • Subject is Microbiology-Biochemistry
    • Subject is Materialforschung Holz
    • Subject is IRTG 2172 - PRoTECT
    • Subject is SensoryMotor Neuroscience
    • Subject is BioNutz-MolwissBiotechnol
    • Subject is Computer Science -PCS-
    • Subject is Umweltinformatik
    • Subject is Geography
    • Subject is Psychologie
    • Subject is Chemie
    • Subject is Emerging Infect. Diseases
    • Subject is Catalysis Sust.Synt.CaSuS
    • Subject is MolecularBiology of Cells
    • Subject is Molekulare Biologie
    • Subject is MetalSites i.Biomolecules
    • Subject is Environmental Informatics
    • Subject is Mathematical Sciences
    • Subject is Molekulare Medizin
    • Subject is Geographie
    • Subject is Geoscience
    • Subject is Behavior and Cognition
    • Subject is Theoret Computat Neurosci
    • Subject is Environmental Informatics
    • Subject is Physik
    • Subject is Biomol:Struc-Func-Dynamic
    • Subject is PhysicsBiologComplexSyste
    • Subject is Cardiovascular Science
    • Subject is Genome Science
    • Subject is Genes in Development, Disease and Evolution
    • Subject is Systems Neuroscience
    • Subject is Biologie
  • Enrolment is allowed for up to 2 courses of the admission set.
  • At least one of these conditions must be fulfilled for enrolment:
    • Degree is Promotion Cotutelle
    • Degree is Promotionsstudiengang
    • Degree is Promotionsprogramm
    • Degree is PromStudiengang Cotutelle
    • Degree is Promotion m.Abschlussprfg
    • Degree is PromProgramm Cotutelle
  • The enrolment is possible from 01.09.2022, 00:00 to 20.09.2022, 23:59.
Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.