Blockveranstaltung: Nerve cell culture and patch-clamp recordings from nerve cells - Details

Blockveranstaltung: Nerve cell culture and patch-clamp recordings from nerve cells - Details

Close message box
You are not logged into Stud.IP.

General information

Course name Blockveranstaltung: Nerve cell culture and patch-clamp recordings from nerve cells
Subtitle
Course number 340038
Semester SoSe 2020
Current number of participants 4
maximum number of participants 12
Home institute Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
participating institutes Georg-August University School of Science (GAUSS) (für Mathematik und Naturwissenschaften)
Courses type Blockveranstaltung in category Teaching
First date Thursday, 16.04.2020 09:00 - 23:59
Type/Form
Participants Target group: GGNB and GAUSS doctoral students.
Pre-requisites Basic theoretical knowledge of nerve cell and synapse function and of patch clamp methodology is desirable.
Learning organisation The course will take place at the Neurophysiology Group, MPI for Experimental Medicine, Hermann-Rein-Str. 3.
ECTS points 1.0

Rooms and times

No room preference
Thursday, 16.04.2020 09:00 - 23:59
Friday, 17.04.2020 09:00 - 23:59

Comment/Description

The course is given by Dr. JeongSeop Rhee: <rhee@em.mpg.de>


To study synaptic transmission mechanisms, my lab takes advantage of the single cell autaptic neuron culture system. This model system is ideally suitable for understanding the most important parameters underlying synaptic communication in a quantitative fashion. It is unique, as all synapses originate from a single axon. Thus different synaptic release modes can be quantified.

Step 1. Preparing autaptic neuron cultures

The autaptic preparation is defined by a single neuron that resides on an island of astrocytes with limited size, called a microisland culture. First, course participants will learn how the micoisland astrocyte culture is made and developed. Second, course participants will learn how to grow single neurons on the astrocyte islands. The applicants will learn to dissociate neurons from target areas of the mouse brain and to culture them on the astrocyte feeder culture.

Step 2. Measuring evoked synaptic transmission in autaptic cultures

In autaptic neuron cultures, all synapses that contact to the dendrite of the neuron are formed by a single axon of the same neuron. Thus, all synapses can be stimulated to release transmitter at once by brief somatic depolarization. To understand the evoked synaptic responses, my lab uses a basic application of the patch clamp technique. Course participants will learn the basics of the measurement and quantification of synaptic responses in autaptic neuron cultures.

This course is intended for students who want to explore projects concerned with synaptic function in neurons.

Admission settings

The course is part of admission "GGNB_Methods_2020_SoSe_February_April".
You can register and sign out for courses in April from 1-20 February. The slots will be assigned around 25 February.
In exceptional circumstances, you can still sign out before 29 February.
Your participation becomes binding on 1 March. Thereafter, cancellation is only possible with a) sick certificate, or b) statement by supervisor. If you do not provide this, you will be banned from courses for the current and next semester, as well as travel grants and other financial support.
By signing up you agree to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account.
The following rules apply for the admission:
  • At least one of these conditions must be fulfilled for enrolment:
    • Degree is Promotionsprogramm
    • Degree is Promotionsstudiengang
    • Degree is PromProgramm Cotutelle
    • Degree is Promotion m.Abschlussprfg
  • The enrolment is possible from 01.02.2020, 00:00 to 20.02.2020, 23:59.
  • At least one of these conditions must be fulfilled for enrolment:
    • Subject is PhysicsBiologComplexSyste
    • Subject is Geography
    • Subject is Mathematical Sciences
    • Subject is Geographie
    • Subject is Environmental Informatics
    • Subject is Biomol:Struc-Func-Dynamic
    • Subject is Chemie
    • Subject is Emerging Infect. Diseases
    • Subject is Microbiology-Biochemistry
    • Subject is Geoscience
    • Subject is Molekulare Biologie
    • Subject is Environmental Informatics
    • Subject is Behavior and Cognition
    • Subject is Biologische Diversität und Ökologie
    • Subject is Genome Science
    • Subject is Theoret Computat Neurosci
    • Subject is Genes in Development, Disease and Evolution
    • Subject is Catalysis Sust.Synt.CaSuS
    • Subject is Neurowissenschaften
    • Subject is IRTG 2172 - PRoTECT
    • Subject is Physik
    • Subject is Umweltinformatik
    • Subject is Molekulare Medizin
    • Subject is Systems Neuroscience
    • Subject is MolecularBiology of Cells
    • Subject is MetalSites i.Biomolecules
    • Subject is Computer Science -PCS-
    • Subject is SensoryMotor Neuroscience
    • Subject is BioNutz-MolwissBiotechnol
    • Subject is Materialforschung Holz
    • Subject is Biologie
    • Subject is MolecularPhysi oftheBrain
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 29.02.2020 on 23:59. Additional seats may be available via a wait list.
  • Enrolment is allowed for up to 2 courses of the admission set.
Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.